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EU funding (€1,500,000): Building emotional circuits: interfacing intrinsic programs with early-life experiences Hor1 Oct 2024 EU Research and Innovation programme "Horizon"
Overview
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Building emotional circuits: interfacing intrinsic programs with early-life experiences
Emotion regulation and responses to fear and stress are vital for survival and interactions with the environment. Within the brain, the amygdala governs emotional states through specialised nuclei and connectivity, controlling anxiety, fear reactions, and emotional learning. In mammals, amygdala circuits mature postnatally and are influenced by mother-offspring bonding, which impairment causes early-life stress and leads to lasting consequences on anxiety. However, the molecular and cellular mechanisms underlying amygdala neuron differentiation and wiring, their reliance on intrinsic programs, potential sex differences, and impact of early-life experiences remain poorly understood. Here, I hypothesise that the differentiation of amygdala neurons relies on transcriptional programs, which vary between sexes and are modulated by early-life stress. Building on advanced technologies and expertise for single-cell gene expression, spatial position and connectivity analyses, that I developed in my prior work, I propose to test this in the amygdalae of female and male mice, by: 1. Identifying transcriptional programs guiding neuron differentiation using single-nucleus and spatial transcriptomics. 2. Characterizing projection development and intrinsic programs using barcoded axon tracings combined with transcriptomics. 3. Determining the role of intrinsic determinants and the impact of early-life stress on projection development and function using CRISPR-Cas9-mediated gene manipulation, maternal separation, 3D axon tracings, and anxiety/emotional memory tests. These experiments will reveal transcriptional programs governing amygdala circuit development and sex dimorphisms. They will identify amygdala neuron types sensitive to environment, potentially more prone to be affected in diseases. This research will contribute to understanding the genetic and environmental aspects of emotional dysregulation, a common feature in neuropsychiatric disorders, including anxiety disorders.
Funded Companies:
| Company name | Funding amount |
| VIB VZW | €1,500,000 |
Source: https://cordis.europa.eu/project/id/101162673
The filing refers to a past date, and does not necessarily reflect the current state. The current state is available on the following page: Vlaams Instituut Voor Biotechnologie - Flanders Institute for Biotechnology VZW, Ghent, Belgium.
