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UK funding (£324,255): Exploring the Potential of Networked Directed Evolution Based on Novel LacI/effector Pairs Ukri15 Jun 2012 UK Research and Innovation, United Kingdom
Overview
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Exploring the Potential of Networked Directed Evolution Based on Novel LacI/effector Pairs
| Abstract | Understanding of the evolution of functional proteins remains a daunting challenge, despite intense research efforts in basic and applied research. Nature's ability to create functional proteins is still unrivalled, raising the question in which respect we can can improve biomimetic efforts in directed evolution to make laboratory evolution more powerful. One key difference is the context in which evolution occurs: laboratory evolution usually deals with enzymes as single species, but natural enzymes are typically embedded in regulated networks and interdependent pathways. This project attempts to build simple networks in which expression of a coupled reporter marks E. coli cells that successfully express a new, functional protein and renders them selectable. This approach could expand the reaction classes that are accessible for directed evolutino, as current laboratory evolution is primarily targeted at functions that are assayed directly, e.g. by generating a fluorescent product. We hope to develop a method that will make directed evolution more versatile and efficient, but also provide insight into how network regulation might have contributed to natural evolution. |
| Category | Research Grant |
| Reference | BB/J008214/1 |
| Status | Closed |
| Funded period start | 15/06/2012 |
| Funded period end | 14/06/2015 |
| Funded value | £324,255.00 |
| Source | https://gtr.ukri.org/projects?ref=BB%2FJ008214%2F1 |
Participating Organisations
| University of Cambridge |
The filing refers to a past date, and does not necessarily reflect the current state. The current state is available on the following page: University of Cambridge, Cambridge.
