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UK funding (£375,895): Dissection of a novel 'periphery to brain' circuit that synchronizes Drosophila's circadian clock with temperature cycles Ukri1 Jan 2010 UK Research and Innovation, United Kingdom
Overview
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Dissection of a novel 'periphery to brain' circuit that synchronizes Drosophila's circadian clock with temperature cycles
| Abstract | Circadian clocks are biological timers that tick in most organisms, including humans. These clocks control a wide array of biological processes, including our sleep/wake cycle, appetite, or body temperature. They function without any input from the outside, meaning they are true clocks that tick with an approximate 24 hr rhythm, even when the organism is kept in total darkness. In other words, human beings keep their daily sleep wake cycles, even when kept in total isolation from the outside world. In nature on the other hand, our circadian clocks are strongly influenced by the environment, as for example by the daily light/dark and temperature changes. As a consequence, circadian rhythms are synchronized with the environment. An impressive example for both the independence of circadian clocks and their ability to communicate with the environment are the phenomena associated with travel across time zones (jetlag) or shift work. If you are 'jetlagged', your circadian clock is still ticking according to the time where you boarded your plane and is telling you to be awake in the middle of the night. Gradually though, your internal clock will adjust (synchronize) to the new time zone and you will feel comfortable again. Molecularly, circadian clocks are assembled by several so called 'clock genes', which are active in certain neurons in the brain and control important biological rhythms. The activity of these clock genes itself is regulated in a rhythmic fashion-they become active in 24 hr periods. The time of maximal or minimal gene activity is determined by the natural light-dark and temperature cycles an organism is exposed to. In other words, the way our clocks are synchronized with the outside world is mediated by directly changing clock gene expression in response to light/dark or temperature changes. The current proposal is aimed to investigate how temperature cycles can synchronize the circadian clock of fruit flies. We are very interested to solve this question, because we discovered that the mechanism involved must be very different from that described for light-synchronization. In flies, the latter is mainly mediated by the blue-light photoreceptor Cryptochrome (Cry), a protein that is expressed within the clock neurons in the fly's brain. As a consequence, the circadian clock of fly brains can be synchronized by light:dark cycles, even when the brains are taken out of the fly and cultured in a dish. Although (in analogy with Cry) we initially expected that the clock neurons also contain a temperature receptor, we found that 'brains in a dish' can not synchronize their clock to temperature cycles! This was a big surprise, indicating that the clock neurons in the brain receive the temperature information from somewhere else in the fly. In this proposal we want to identify these cells or organs, and we already have some promising preliminary findings: Previously we had isolated 'nocte' as temperature synchronization mutant. When we reduce the function of the 'nocte' gene in peripheral sensory organs, we can destroy the fly's ability to synchronize to temperature cycles. This means we now have a gene and candidate sensory structures at hand, which will allow us to start unravelling the temperature synchronization pathway. We will also investigate a class of ion channels (Trp channels), that has been shown to function as 'environmental sensors' in both vertebrates and insects. They can, for example, mediate responses to extreme temperatures, touch, or hot chilli peppers. We will test if these channels are important for temperature synchronization by analysis of available Trp channel mutants. Finally, we want to identify proteins that interact with Nocte by applying a modern proteomics purification approach involving Mass-spec analysis. By doing this, we hope to identify additional factors that will help to resolve the temperature synchronization pathway in flies. |
| Category | Research Grant |
| Reference | BB/H001204/1 |
| Status | Closed |
| Funded period start | 01/01/2010 |
| Funded period end | 14/06/2013 |
| Funded value | £375,895.00 |
| Source | https://gtr.ukri.org/projects?ref=BB%2FH001204%2F1 |
Participating Organisations
| Queen Mary University of London | |
| University of Bristol |
The filing refers to a past date, and does not necessarily reflect the current state. The current state is available on the following page: Queen Mary University of London, London.
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